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. 2011 Jul 7;286(35):30415–30422. doi: 10.1074/jbc.M111.268433

FIGURE 8.

FIGURE 8.

Correlation between cross-linking and transport activity of F29C/V158C in RSO. Cross-linking experiments and [1-14C]lactose transport assays were performed as described in the legend to Fig. 3. A, Western blots. B, active transport. ■, control; ●, in the presence of 0.5 mm Cu/Ph; ▴, in presence of 0.5 mm Cu/Ph and 10 mm TDG; ▾, vector pT7-5. C, active transport of F29C/V158C after cross-linking with (MTS)2-1 as assayed identically as in B: ■, control; ●, in the presence of 0.05 mm (MTS)2-1; ▴, in the presence of 0.05 mm (MTS)2-1 and 10 mm TDG; ▾, vector pT7-5. D, mutation V158C and a Cys replacement for Phe29 are denoted by sticks based on the structure of LacY (PDB code 2V8N).