FIGURE 1.

Growth impairment of strains defective in peroxisomal targeting signal receptors on glucose minimal medium. A, growth of the A. oryzae ΔAopex5 and ΔAopex7 strains on minimal media (CD + Met) containing either glucose or acetate or oleic acid as the sole carbon source, and nutrient-rich medium (DPY). Conidial solutions (10³ conidia/5 μl) of the wild-type, ΔAopex5, and ΔAopex7 strains and the complemented strains were spotted on each medium (as indicated to the right) and incubated at 30 °C for 3 days. B, swollen morphology of germinated conidia of the ΔAopex5 and ΔAopex7 strains in glucose minimal medium. Conidia of the wild-type, ΔAopex5, and ΔAopex7 strains were inoculated in minimal medium (CD + Met) containing glucose at a concentration of 10³ conidia/100 μl. The cultures were incubated at 30 °C for 7 h (wild-type) or 15 h (ΔAopex5 and ΔAopex7) and observed by microscopy. Note that a longer incubation time was required for the ΔAopex5 and ΔAopex7 strains to germinate. Bars: 5 μm.