FIGURE 3.

Deletion of PTS1 from BioF protein leads to biotin auxotrophy on glucose minimal medium. A, growth of the ΔAobioF and AobioFΔPTS1 strains on minimal medium (M + Met) containing either glucose or acetate as the sole carbon source supplemented with biotin. Conidia solutions (10³ conidia/5 μl) of the strains were spotted on plates (as indicated to the right) and incubated at 30 °C for 3 days. B, swollen morphology of germinated conidia of the ΔAobioF and AobioFΔPTS1 strains in glucose minimal medium. Conidia of these strains were inoculated in minimal medium (CD + Met) containing glucose and 0.5% casamino acids at a concentration of 10³ conidia/100 μl. Photographs of germinated conidia were taken after the cultures were incubated at 30 °C for 20 h. Bars: 5 μm.