FIGURE 3.
LRP1 binds to immobilized αM-I domain. A, microtiter wells were coated with the αM-I domain, blocked with BSA, and then incubated with increasing concentrations of purified human LRP1. The amount of LRP1 bound following washing was determined using monoclonal antibody 8G1. As a control, the binding of LRP1 to BSA-coated wells was also measured. The solid curve represents the best fit to a binding isotherm as determined by nonlinear regression analysis. B, binding of monoclonal antibodies LM2/1 and 44A to microtiter wells coated with the αM-I domain (closed symbols) or BSA as a control (open symbols). Data shown are representative of two independent experiments.