FIGURE 7.

LRP1 modulates α_Mβ₂ function. A, 293 cells stably transfected with α_Mβ₂ were incubated in the absence or presence of sLRP1 (20 nm). Following incubation, the ability of the cells to adhere to fibrinogen-coated wells was measured. *, p = 0.003. B, primary macrophages isolated from LRP1^+/+ or macrophage-specific LRP1-deficient mice (macLRP1^−/−) mice were analyzed for LRP1 expression by immunoblot analysis using antibody R2629. C, α_Mβ₂ internalization upon incubation of macrophages with LPS (10 ng/ml) at 37 °C was measured by FACS analysis using an FITC conjugate of anti-rat IgG. The mean fluorescence intensity at time 0 was assigned 100%. The data shown represent the means ± S.D. of a duplicate experiment. *, p < 0.05.