FIGURE 3.

mKO mice display normal activation of exercise-responsive proteins following AEX. Protein phosphorylation and total abundance were determined in gastrocnemius whole muscle lysates of sedentary (SED) mice or immediately after AEX (0 h post). A–C, phosphorylation of AMPK^Thr172 and its substrates (A), ACCβ^Ser79 (B), and HDAC5^Ser498 (C) were increased equally 0 h after AEX versus SED, in WT and mKO. D and E, phosphorylation of ATF2^Thr71 and CREB^Ser133 (D) and CaMKII^Thr286 and p38 MAPK^{THR180/TYR182} (E) were increased equally 0 h after AEX versus SED in WT and mKO. Data represent n = 4–6/group. All data are presented as mean ± S.E. (error bars). *, within genotype p < 0.05.