FIGURE 5.

PGC-1α nuclear translocation and deacetylation following AEX are unaffected by loss of SIRT1 activity. A and B, integrity of nuclear and cytosolic fractions was determined via immunoblotting for lactate dehydrogenase (LDH) (cytosolic; Cyto) and histone H2B (nuclear; Nuc) abundance. PGC-1α nuclear abundance increases 0 h (A) and 3 h (B) after AEX (0h Post and 3h Post, respectively) compared with sedentary (SED) in both mKO and WT mice. C and D, to determine PGC-1α acetylation, we immunoprecipitated (IP) with an antibody to acetylated proteins (Ac-Lys) or PGC-1α and immunoblotted (IB) for Ac-PGC-1α or total PGC-1α. PGC-1α was deacetylated to the same extent 0 h (C) and 3 h (D) after AEX in mKO and WT mice compared with SED mice. Data represent n = 4–6/group. All data are presented as mean ± S.E. (error bars). *, within genotype p < 0.05.