FIGURE 6.

Nuclear abundance of GCN5 and the association of GCN5 with PGC-1α are decreased after AEX. A, immunoblotting (IB) of GCN5 and H2B in nuclear fractions of sedentary (SED) and 3 h after AEX (3h Post) skeletal muscle of WT and mKO mice. 3 h after AEX the nuclear abundance of GCN5 is decreased in both mKO and WT mice compared with SED mice. B, significant increase in the nuclear PGC-1α to GCN5 ratio in both WT and mKO mice 3 h after AEX. C, immunoprecipitation of GCN5 or PGC-1α and immunoblotting of GCN5 or PGC-1α demonstrate that GCN5 and PGC-1α interact in vivo and that this interaction is reduced 3 h after AEX in both mKO and WT mice compared with SED mice. Data represent n = 4–6/group. All data are presented as mean ± S.E. (error bars). *, within genotype p < 0.05.