Overexpression of 14q32.2 miRNA cluster in HCC is associated with high serum AFP value and poor overall survival of liver cancer patients. a, analysis of variance box-and-whisker plots comparing serum AFP levels (ng/ml) (y axis) in two groups of patients. 97 HCC patients are divided into two groups by 14q32.2 microRNA expression levels; 79 show no overexpression, and 18 show overexpression. The significance of the difference in AFP level distributions is given above the plot. b, Kaplan-Meier plots comparing survival times of two groups of patients with overexpression and without overexpression of 14q32.2 miRNAs. c, in vitro wound healing assay showing that miRNAs from the DLK1-DIO3 cluster enhanced HCC cell migration potential. The PLC/PRF/5 hepatoma cell line was transfected with the selected miRNAs (miR-127, miR-431, and miR-433) as indicated or control vector. The migration of cells toward the wound was monitored, and images were captured at time 0 and 24 h. Magnification, ×100.