FIGURE 2.

Gα₁₃QL activates one or more forms of Gli in C3H10T1/2 cells. C3H10T1/2 cells were transfected with vectors for constitutively active Gα family members (QL mutants), SmoM2 or Gli2, or empty vector, together with 8xGli firefly and TK Renilla luciferase reporters. At 48 h, when cells had reached confluence, the serum was lowered to 0.5%. Luciferase activities were assayed 24 h later. The data are means of firefly/Renilla activities normalized to that obtained for pcDNA from three to 36 independent experiments carried out in triplicate. Inset, experiments were carried out in the same manner, but comparing normal with mutated 8xGli reporter plasmids. Values are means of six independent experiments carried out in triplicate. Differences from pcDNA were evaluated using paired t tests. *, p < 0.05, and **, p < 0.01.