FIGURE 4.

Activation of Gli(s) is most evident for Gα₁₃QL among variants of the G_12/13 family. A, C3H10T1/2 cells were transfected with (left) empty vector or vectors for wild type or constitutively active Gα₁₂ or Gα₁₃, or Gli2, or (right) with empty vector or constitutively active RhoA (G14V) or Gα₁₃QL, as well as with 8xGli firefly and TK Renilla luciferase reporters. B, C3H10T1/2 cells were transfected as in A, except substituting an SRF reporter for the 8xGli firefly reporter. The data are means of ratios of firefly/Renilla activities normalized to those obtained for pcDNA from four or five independent experiments carried out in triplicate. Differences from pcDNA were evaluated using paired t tests. *, p < 0.05; **, p < 0.01.