FIGURE 6.

Activation of the bcl-2 promoter by Gα₁₃QL requires Gli1. A, L3.6 cells were transfected with Gα₁₃QL, or empty vector (pCMV), together with a luciferase reporter containing the bcl-2 promoter and either a control (scrambled) or Gli1-targeted shRNA. 36 h after transfection, cells were harvested. Data are means of luciferase to protein ratios normalized to that of vector alone for four experiments carried out in triplicate. Statistical differences were calculated using paired t tests. **, p < 0.01. B, L3.6 cells were transfected with Gα₁₃QL, or empty vector (pCMV), together with control (scrambled) or Gli1-targeted shRNA. Levels of Gli1 were evaluated at 36 h by means of Western blotting. Shown is one of two experiments with identical results.