FIGURE 7.
CCKA activates Gli(s). A, PANC1 cells were transfected with CCKA, or empty vector (pCMV), together with the 8xGli reporter gene as described in the legend to Fig. 5. Data are means of luciferase to protein ratios normalized to those of pCMV for four independent experiments carried out in triplicate. The difference from pCMV was evaluated using a paired t test. *, p < 0.05. B, AR42J cells were transfected with the same reporter gene. After 36 h, medium was replaced with serum-free medium supplemented, or not (vehicle), with 100 nm CCK-8. Luciferase activity and total protein were assayed 12 h later. Data are means of ratios of luciferase/protein normalized to that for pCMV from six independent experiments carried out in triplicate. Difference from vehicle was evaluated using a paired t test. *, p < 0.05.