FIGURE 8.

Activation of the Gli reporter by Gα₁₃QL can be inhibited by SB202190, LFM-A13, and KN compounds. C3H10T1/2 cells were transfected with Gα₁₃QL or empty vector (pcDNA), together with 8xGli firefly or TK Renilla luciferase reporters. 24 h after transfection, the serum was lowered to 0.5% and supplemented with 10 μm SB202190 or vehicle (A); 100 μm LFM-A13 or vehicle (B); or 5 μm KN-93, 5 μm KN-92, or vehicle (C). Luciferase activities were assayed 24 h later. The data are means of ratios of firefly/Renilla activities normalized for Gα₁₃QL without treatment from three to eight independent experiments carried out in triplicate. Significance was determined using paired t tests. **, p < 0.01; ***, p < 0.001; ns, not significant.