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. 2011 Jun 16;286(35):30795–30805. doi: 10.1074/jbc.M111.255984

FIGURE 3.

FIGURE 3.

Loss of α-actinin-4 diminishes the level of CLP36 in podocytes. Human podocytes were transfected with α-actinin-4 siRNA A-KD1 or A-KD2 (A and B), CLP36 siRNA C-KD1 or C-KD2 (C and D), or a control RNA as indicated. The densities of the α-actinin-4 band in the A-KD1 and A-KD2 transfectants and those of the CLP36 band in the C-KD1 and C-KD2 transfectants were quantified using National Institutes of Health ImageJ program and compared with those of control transfectants. Under the conditions used, the levels of α-actinin-4 in the A-KD1 and A-KD2 transfectants were 23 and 34% of that of the control transfectants, and the levels of CLP36 in the C-KD1 and C-KD2 transfectants were 12 and 25% of that of the control transfectants. Total cell lysates (A and C), Triton X-100-soluble and -insoluble fractions (B and D) were prepared as described under “Experimental Procedures.” The samples were analyzed by Western blotting with Abs for α-actinin-4, CLP36,, or tubulin (as a loading control) or Coomassie Blue staining (to show equal loading).