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. 2011 Jun 16;286(35):30795–30805. doi: 10.1074/jbc.M111.255984

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — FSGS-associated R310Q or Q348R mutation inhibits α-actinin-4 interaction with CLP36. Human podocytes were transfected with expression vectors encoding FLAG-tagged wild-type or α-actinin-4 mutants bearing R310Q (A), Q348R (B), or K255E (C) mutation. CLP36 was immunoprecipitated from cell lysates with an anti-CLP36 Ab. The lysates (lanes 1 and 2) and immunoprecipitates (lanes 3 and 4) were analyzed by Western blotting with anti-FLAG and anti-CLP36 Abs as indicated. We have performed two independent immunoprecipitation experiments and obtained similar results from both experiments.