FIGURE 7.

Disruption of the α-actinin-4-CLP36 complex reduces RhoA activity and traction force in podocytes. Human podocytes were infected with adenoviral vectors encoding FLAG-ΔLIM or β-galactosidase (as a control) as in Fig. 4. A, active RhoA measured as described under “Experimental Procedures.” Bars represent means ± S.D. (error bars) from three independent experiments. *, p < 0.05 versus the control. B, representative images of traction force microscopy of podocytes infected with the control or FLAG-ΔLIM adenoviral vectors. C, bars represent relative cell traction force (CTF) (means ± S.D.) from three independent experiments. *, p < 0.05 versus the control.