FIGURE 4.

NRVM treated with PI3K(p110α) inhibitors (PIK75 or A66) in the absence and presence of IGF1. A and B, Western blot analyses of pAkt, total Akt, pERK, and ERK in cell lysates from NRVM treated with or without IGF1 in the presence of PIK75 (0–100 μm) or A66 (0–50 μm). C and D, immunofluorescence of control NRVM (dimethyl sulfoxide, DMSO) and NRVM treated with PIK75, A66, and/or IGF1 for 18 h. Anti-α actinin (a–f) stains sarcomeric bands and Z-lines, phalloidin-FITC (g–l) stains contractile thin filaments, and DAPI (m–r) stains nuclei. s–x, merged images. Scale bar = 20 μm. Experiments were repeated twice with identical findings. Representative images from a single experiment are shown.