FIGURE 3.

ATP binding cassette A1 transporter expression is induced by IL-6 in human THP-1 macrophages. Human THP-1 macrophages were treated with or without IL6 (50 ng/ml) ± TO901317 (1 μm) for 24 h, and ABCA1 expression was assessed by quantification of mRNA by real-time quantitative PCR (A) and protein levels by Western blot (B). Levels of ABCA1 mRNA were normalized to housekeeping genes (δ-aminolevulinate synthase and human α-tubulin) and ABCA1 protein to matching annexin1 levels. Values are the means ± S.E. of three independent experiments. *, p < 0.05; **, p < 0.001 versus untreated control cells; †, p < 0.05; ††, p < 0.001 versus IL-6-treated cells; ‡, p < 0.001 versus TO901317-stimulated cells.