FIGURE 3.
A, levels of GlgP (Sll1356) and GlgX (Slr0237 and Slr1857) proteins in the GT, G50, and GOX50 strains under light and dark conditions. Total protein (16 μg) was subjected to immunoblotting. Data represent means ± S.D. of values from seven or five independent experiments of immunoblotting with antisera against GlgP (Sll1356) and GlgX (Slr1857), respectively. The levels were calibrated relative to the value obtained in the GT strain under light conditions, which was set at 100%. Differences between GT and G50 or GOX50 analyzed using Student's t test were statistically significant (*, p < 0.05; **, p < 0.005). B, levels of glycogen in the GT and GOX50 strains under light and dark conditions. Data represent means ± S.D. of values from three independent experiments. The levels were calibrated relative to the value obtained in the GT strain under light conditions, which was set at 100%. Differences between GT and GOX50 analyzed using Student's t test were statistically significant (*, p < 0.05; **, p < 0.005).