FIGURE 1.

Consensus amino acid sequences of gPres and A4 and diagram of the construction of the chimeric pendrin containing the 14-amino acid motif from gPres. A, 14 amino acids from gPres were used to replace 20 amino acids in A4 to generate A4(g14). The amino acid residues being swapped between gPres and A4 are highlighted by the red box. Identical residues in the consensus sequences are shown in red. B, schematic drawings of the constructs of gPres, A4, and A4(g14). After substitution, A4(g14) has 774 amino acids (a.a.).