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. 2011 Jul 13;286(35):31014–31021. doi: 10.1074/jbc.M111.259564

FIGURE 2.

FIGURE 2.

Heterogenic expression and NLC measured from gPres, A4, and A4(g14). A, representative confocal images of attached and detected HEK cells transfected by A4 and A4(g14) with EGFP tag (in green). The membrane of transfected cells was labeled by di-8-ANEPPS (di-8, in red), a membrane-bound dye. Co-localization of fluorescent signals indicates the membrane-associated expression of the proteins (merge, orange). Bar: 10 μm. B, examples (color-coded thin lines) of NLC obtained from HEK cells transfected with gPres, A4, and A4(g14), respectively. The capacitance-voltage responses were fitted with the Boltzmann function (color-coded heavy lines). Clin was subtracted. pF, picofarads. C, four parameters derived from curve fittings with Boltzmann's function. Data are expressed as mean ± S.D. n = 18, 13, and 14 for gPres, A4, and A4(g14), respectively. *, p < 0.05, **, p < 0.01 (Student's t test).