Table 1.
Up-regulation proteins in the endometrium of non-pregnant pigs
| Spot No. |
Protein Name | NCBI Accession No. | SwissProt Accession No. | Method of ID | Score | Peptides Matched | Peptides Obtained | Sequence Coverage (%) | MW [Da] |
PI | MS/Ms Score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | SEPT2 protein | gi|23274163 | Q15019 | A | 100 | 13 | 79 | 45 | 42,886 | 6.38 | |
| 2 | Transgelin | gi|48255905 | Q01995 | A | 77 | 9 | 37 | 36 | 22,828 | 8.01 | |
| 3 | Transgelin | gi|48255905 | Q01995 | A | 95 | 9 | 25 | 48 | 22,828 | 8.01 | |
| 4 | Transgelin | gi|48255905 | Q01995 | A, B | 90 | 12 | 36 | 54 | 22,828 | 8.01 | 53 |
| 5 | Transgelin | gi|48255905 | Q01995 | A, B | 72 | 8 | 32 | 44 | 22,828 | 8.01 | 41 |
| 6 | Transgelin | gi|48255905 | Q01995 | A | 70 | 5 | 26 | 55 | 22,828 | 8.01 | |
| 7 | Transgelin | gi|48255905 | Q01995 | A | 83 | 10 | 49 | 44 | 22,828 | 8.01 | |
| 8 | Protein DJ-1 | gi|56404943 | Q99497 | A | 64 | 7 | 36 | 42 | 20,050 | 6.33 | |
| 9 | Cofilin 1 | gi|5031635 | P23528 | A | 201 | 7 | 65 | 54 | 18,719 | 8.22 | |
| 10 | Fascin 1 | gi|4507115 | Q16658 | A | 198 | 24 | 98 | 51 | 55,123 | 6.84 | |
| 11 | Cellular retinoic acid binding protein 1 | gi|4758052 | P29762 | A, B | 105 | 10 | 88 | 67 | 15,727 | 5.3 | 67 |
| 12 | Transferrin | gi|833800 | B3CL06 | A | 74 | 15 | 68 | 24 | 78,954 | 6.76 | |
| 13 | Transferrin | gi|833800 | B3CL06 | A, B | 89 | 11 | 50 | 19 | 78,954 | 6.76 | 57 |
| 14 | Transferrin | gi|833800 | B3CL06 | A | 119 | 15 | 33 | 28 | 78,954 | 6.76 | |
| 15 | Transferrin | gi|833800 | B3CL06 | A, B | 118 | 16 | 54 | 27 | 78,954 | 6.76 | 29 |
| 16 | Smooth muscle protein | gi|177175 | P62736 | A | 81 | 10 | 65 | 54 | 22,518 | 8.56 | |
| 17 | Smooth muscle protein 22-alpha [Fragment] | gi|75038933 | O62766 | A | 68 | 4 | 33 | 70 | 10,156 | 4.93 | |
| 18 | Smooth muscle gamma-actin | gi|950002 | P63267 | A, B | 95 | 9 | 48 | 40 | 43,251 | 5.36 | 108 |
| 19 | Smooth muscle protein | gi|177175 | P62736 | A | 108 | 12 | 59 | 55 | 22,518 | 8.56 | |
| 20 | Nck-associated protein 5 isoform 1 | gi|126362961 | O14513 | A | 70 | 20 | 73 | 14 | 211,001 | 7.17 | |
| 21 | Alpha-actin | gi|49870 | P68137 | A, B | 83 | 11 | 64 | 48 | 39,465 | 5.83 | 118 |
| 22 | Beta-actin | gi|9864780 | P60709 | A | 75 | 8 | 69 | 42 | 32,202 | 5.15 | |
| 23 | Gamma-actin | gi|809561 | P63260 | A | 75 | 9 | 50 | 44 | 41,335 | 5.56 | |
| 24 | Similar to actin alpha 1 skeletal muscle protein isoform 1 | gi|114631697 | P68133 | A | 78 | 9 | 63 | 42 | 29,335 | 5.67 | |
| 25 | Similar to Uncharacterized protein C9orf68 | gi|149736845 | Q8N4H0 | A | 70 | 7 | 25 | 22 | 46,046 | 9.35 | |
| 26 | Beta-globin | gi|120564455 | P68871 | A | 82 | 6 | 52 | 42 | 14,010 | 6.04 | |
| 27 | TRIMCyp | gi|76576111 | B0ZE27 | A | 76 | 8 | 21 | 43 | 18,194 | 8.26 | |
| 28 | Similar to actinin, alpha 4 isoform 13 | gi|73947742 | O43707 | A | 73 | 9 | 32 | 14 | 108,550 | 5.3 | |
| 29 | Hsp 90 beta | gi|20149594 | P08238 | A | 82 | 4 | 13 | 24 | 83554 | 4.97 |
Method of ID: A) MALDI-TOF, B) MALDI-TOF/TOF MS.