Table 1.
In vitro inhibitory effect (expressed as IC50) of the antibiotics tested against PAF-induced aggregation of WRPs and their ability to induce platelet aggregation
| IC501 towards PAF in WRPs (μg/mL) | ||||||
|---|---|---|---|---|---|---|
| Bioactive Compound |
Median | Min | Max | Geometric Mean | 95% Confidence Interval | Drug-induced WRPs aggregation/desensitization |
| Clarithromycin | 0.18 | 0.14 | 0.28 | 0.19 | 0.08 thru 0.46 | -/- |
| Azithromycin | 0.40 | 0.20 | 0.85 | 0.41 | 0.07 thru 2.46 | -/- |
| Linezolid | 1.25 | 0.60 | 1.62 | 1.07 | 0.30 thru 3.84 | -/- |
| Amikacin | 2.73 | 1.50 | 4.55 | 2.65 | 0.67 thru 10.54 | -/- |
| Netilmicin | 2.80 | 1.45 | 4.70 | 2.67 | 0.62 thru 11.56 | -/- |
| Daptomycin | 5.01 | 2.88 | 7.22 | 4.71 | 1.49 thru 14.85 | -/- |
| Piperacillin/Tazobactam | 17.65/2.22 | 12.18/1.54 | 22.27/2.85 | 16.85/2.14 | 7.91 thru 35.90/0.99 thru 4.61 | -/- |
| Ceftazidime | 30.06 | 20.92 | 37.95 | 28.79 | 13.66 thru 60.68 | -/- |
| Tigecycline | 113.45 | 91.86 | 131.07 | 110.95 | 71.16 thru 173.0 | -/- |
| Vancomycin | ND | - | - | - | - | +/- |
| Meropenem | ND | - | - | - | - | -/- |
Experiments were conducted three times using different platelets preparations. 1IC50 values are expressed as μg/mL of bioactive compound in the aggregometer cuvette, Final concentration of PAF in the aggregometer cuvette when tested in WRPs was 4.4 × 10-11M. WRPs: Washed Rabbit Platelets; ND: Not detected inhibition against PAF-induced platelet aggregation; -/-: Not detected platelet aggregation; +/-: Detected platelet aggregation/not detected platelet desensitization against PAF.