Table 2.

Reaction inhibition, fluorescent signal, and melting temperature data: tropomyosin amplicon

Dye	Optimal Concentration	Ct: 0.64 μM (0.32×)	Ct: 2 μM (1×)	Ct: 10 μM (5×)	Ct: 20 μM (10×)	Maximum Fluorescence	Tm shift slope (R2)
SYBR Green*	0.64 μM	33.00 ± 0.10	35.07 ± 1.10	no Ct	no Ct	1.00	3.99, × conc. 1.99, μM conc. (0.97)
EvaGreen*	5×	36.37 ± 0.48	35.04 ± 0.35	35.15 ± 1.13	not determined	0.91	0.36 (0.98)
Green*
SYTO 11	2 μM	38.02 ± 0.97	36.42 ± 0.84	38.17 ± 1.03	42.27 ± 0	0.51	-0.20 (0.12)
SYTO 13	10 μM	37.58 ± 1.33	36.10 ± 1.32	36.06 ± 1.55	36.45 ± 2.06	0.87	0.001 (0.00)
SYTO 16	10 μM	37.00 ± 1.86	34.20 ± 1.05	34.04 ± 0.96	34.97 ± 0.53	1.07	0.030 (0.004)
SYTO 21	0.64 μM	34.55 ± 1.07	35.90 ± 0.83	no Ct	no Ct	0.82	0.62 (0.03)
SYTO 24	0.64 μM	33.50 ± 0.01	no Ct	no Ct	no Ct	1.02	not determined
Orange*
SYTO 80	20 μM	39.16 ± 2.44	37.33 ± 1.86	35.84 ± 0.02	34.78 ± 0.08	0.40	0.048 (0.008)
SYTO 81	20 μM	43.89 ± 0.10	40.62 ± 1.39	37.02 ± 0.39	36.45 ± 0.21	0.11	0.026 (0.002)
SYTO 82	20 μM	37.18 ± 1.85	35.59 ± 0.95	33.50 ± 0.11	33.44 ± 0.51	0.61	-0.17 (0.10)
SYTO 83	20 μM	42.20 ± 0.01	39.28 ± 0.99	37.04 ± 0.13	35.92 ± 0.93	0.10	0.037 (0.0051)
Red*
SYTO 17	20 μM	no Ct	42.26 ± 0.02	36.18 ± 0.14	35.32 ± 0.41	0.03	0.0009 (0.0005)
SYTO 59	10 μM	35.63 ± 0.09	35.15 ± 1.50	33.70 ± 0.10	35.53 ± 0.53	0.20	0.11 (0.83)
SYTO 60	2 μM	34.85 ± 0.41	34.29 ± 0.31	41.09 ± 1.97	no Ct	0.13	0.064 (0.10)
SYTO 61	10 μM	37.45 ± 1.68	35.38 ± 0.57	34.14 ± 0.98	35.00 ± 1.19	0.14	0.043 (0.58)
SYTO 62	0.64 μM	33.98 ± 0.42	33.69 ± 0.75	no Ct	no Ct	0.17	0.035 (0.008)
SYTO 63	2 μM	34.78 ± 0.41	34.50 ± 0.31	37.07 ± 0.07	no Ct	0.12	0.24 (0.85)
SYTO 64	2 μM	36.22 ± 0.10	36.68 ± 1.62	36.31 ± 1.05	36.48 ± 0.95	0.05	-0.004 (0.013)

No fluorescent signals were obtained for SYTO dyes 12, 14, 25, 84, or 85

*C_t values are average ± SD for at 2-3 independent experiments