Figure 1.

COL1A2, ALP, OPG and gut hormone expression profiles. After extraction and reverse transcription, mRNA levels were analysed. Results are expressed as means of at least six replicates per cell line ±SEM. β-actin was used as the housekeeping gene to normalize the levels of mRNA expression. In all the cases these determinations showed highest expression in Saos-2 and the lowest rate was for MG-63. Water was used as non-template control. COL1A2, ALP and OPG had their highest expression in Saos-2, and the lowest in MG-63. GIP-R, GHS-R, and GPR39 mRNA expression was found in the three osteoblastic cell lines. GLP-1R and GLP-2R expression was observed only in MG-63 and TE-85.