Figure 3. myo-Inositol and scyllo-inositol transport in HEK293 cells.

myo-Inositol testing was conducted in HEK293 cells, by incubating cells for 15 minutes in medium containing 100 µM of myo-inositol-(2-³H) in the presence of increasing concentrations of cold myo-inositol (A). Addition of 10 mM of cold myo-inositol, the concentration used in the competitive transport assays, resulted in a near maximal inhibition of myo-inositol-(2-³H) transport in these cells. scyllo-Inositol testing was conducted over a period of 3 hours in these cells, due to slower uptake rates (B). Once again, 10 mM of cold scyllo-inositol produced near maximal inhibition of scyllo-inositol-(2-³H). (n = 3 wells per concentration).