Figure 7. Transport of scyllo-inositol derivatives by SMIT1/2.

Further testing of SMIT1/2 myo-inositol transport inhibition was conducted using derivatives of scyllo-inositol, in which one or two hydroxyl groups were removed or substituted (A). For the control experiment, myo-inositol-(2-³H) transport was quantified in the absence of any competitors. An analysis of myo-inositol-(2-³H) transport in the presence of each of the compounds, found monodeoxy- but not dideoxy-scyllo-inositol competed for myo-inositol-(2-³H) transport (B). Single hydroxyl group substitutions were tolerated in the order chlorine>fluorine>nitrate>hydroxy methyl. (n = 3 wells per variable).