Figure 5. Cell cycle analysis with propidium iodide (PI) and EdU incorporation.

Human MSCs were incubated with polybrene for 24 hr and then cultured for another 4 days in 100 mm dishes with 7 mL of medium per dish. (A) Cell cycle analysis with PI of hMSCs at different polybrene concentrations in the absence or presence of FGF-2. (B) Sample cell cycle histograms of hMSCs incubated in the absence of FGF-2 at 0 and 8 µg/mL of polybrene. (C) Proliferation measurement by EdU incorporation for hMSCs treated and untreated with FGF-2 at different polybrene concentrations (*p<0.001 vs. 0 µg/mL and 1 µg/mL, Tukey t-test). Values are mean ± SD.