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. 2011 Aug 26;6(8):e23891. doi: 10.1371/journal.pone.0023891

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Lin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Human MSCs were cultured with lentivirus containing a dual reporter gene that encodes a fusion protein of luciferase and mRFP. (A) Positive correlation of transduction efficiency with polybrene concentration as determined by mRFP flow analysis. (Spearman corr. coeff. = 0.95, p<0.001) Values are mean ± SD. (B) Image of the bioluminescence signal from the transduced cells. (C) Chart of the number of photons detected in each well as a function of polybrene concentration. (Spearman corr. coeff. = 0.97, p<0.001) Values are mean ± SD.