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. 2011 Aug 26;6(8):e24224. doi: 10.1371/journal.pone.0024224

Figure 2. Arctigenin (ATG) increased PGC-1α transcription via enhancing AMPK phosphrylation.

Figure 2

A. H9C2 cells and differentiated C2C12 cells were cultured with arctigenin or DMSO for 24 hours before harvest. Total RNA extraction, cDNA preparation and PGC-1α mRNA quantification were performed as “Experimental Procedures”. GAPDH mRNA was used as an internal control and data was shown as folded changes of blank control. B. C. H9C2 cells (B) and differentiated C2C12 cells (C) were treated with or without 20 µM compound C for 1 hour before and during the incubation with actigenin (20 µm) for 24 hours. After harvest, phospho- and total AMPK protein levels were analyzed. The bands were quantified using Image-Pro Plus software. Values are means ± SE. D. E. H9C2 (D) and differentiated C2C12 cells (E) were treated with or without 20 µM compound C for 1 hour before and during the incubation with actigenin (20 µM) for 24 hours. Total mRNA was extracted and PGC-1α mRNA level quantified. The results shown are representative of three independent experiments. *, p<0.05; **, p<0.01; ***, p<0.005; one-way ANOVA. #, p<0.05; ###, p<0.005: for compound C and arctigenin co-incubation group versus arctigenin treated group; student's t test.