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. 2011 Aug 26;6(8):e24224. doi: 10.1371/journal.pone.0024224

Figure 3. Arctigenin (ATG) promoted mitochondrial biogenesis and FAO related gene expression.

Figure 3

A. B. H9C2 and differentiated C2C12 cells were treated with indicated concentration of arctigenin (1, 10, 40 µM) or DMSO for 24 hours before harvest. Total RNA extraction, cDNA preparation and ERRα, cytochrome c, SCD1, PDK4, FAS, and mCPT1b mRNA quantification were performed as “Experimental Procedures”. GAPDH RNA was used as an internal control for calculating mRNA fold changes. The results shown are validated by three independent experiments. *, p<0.05; **, p<0.01; ***, p<0.005; one-way ANOVA.