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. 2011 Aug 26;6(8):e24224. doi: 10.1371/journal.pone.0024224

Figure 4. Arctigenin (ATG) enhanced AMPK phosphorylation through CaMKK and LKB1-dependent pathway.

Figure 4

A. Arctigenin was pre-incubated with AMPK α2β1γ1 for 30 min, AMPK activity was measured by monitoring the produced ADP with A-769662 as a positive control. B. HEK293T cells were treated with arctigenin (10 µM) in the absence or presence of STO-609 (2 µg/ml, pre-incubation for 4 h in serum-free DMEM) for 30 min in serum-free DMEM. AMPK phosphorylation and total AMPK levels were determined by western blotting. C. After transfected with pSuper.neo.gfp-LKB1 for 48 hours, HEK293T cells were treated with arctigenin (20 µM) for 30 min. AMPK phosphorylation and total AMPK levels were determined by Western blotting. The bands were quantified using Image-Pro Plus software. Values are means ± SE. Values are means ± SE. The results shown are representative of three independent experiments. *, p<0.05; **, p<0.01; ***, p<0.005; student's t test.