Figure 5. Arctigenin (ATG) elevated mice treadmill performance without inducing myofiber type conversion in skeletal muscle.

A. B. After mice were administrated with arctigenin (8 mg/kg) or vehicle via intraperitoneal for a period of 6 weeks, they were run to fatigue on a treadmill as “Experimental Procedures”. Running time and distance were recorded (n = 8/group). Values are means ± SD. C. D. Total RNA was extracted from gastrocnemius and quadriceps, and type I and type II MHCs were then analyzed by real-time PCR assays (n = 10/group). GAPDH RNA was used as an internal control for calculating mRNA fold changes. ***, p<0.005; student's t test.