Fig. 4.

Representative confocal immunofluorescence images of nmMyoIIA signal (red channel). The arrows point at typical nmMyoIIA signal in the apical domain. Yellow boxes are examples of typical apical (a ROI) and basal (b ROI) regions of interest used for quantification of the signal (Fig. 5). DAPI (blue channel) was used to counterstain DNA. Bar, 20 μm