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. 2011 Jun 22;47(2):157–165. doi: 10.1530/JME-11-0030

Figure 4.

Figure 4

The definition of macrophage subtypes in adipose tissue from non-pregnant and pregnant mice. (A) Identification of F4/80+ CD11c+ macrophages by immunofluorescence microscopy in the frozen sections of SAT stained with antibodies against F4/80 (left) and CD11c (middle) and imaged by confocal microscopy to identify surface markers to confirm the presence of CD11c+ cells. F4/80+ CD11c+ macrophage: M1 macrophage; F4/80− CD11c+ macrophage: M2 macrophage (n=6 per group). (B) The ratio of M1–M2 macrophages in SAT (n=6 per group). (C) Identification of F4/80+ and CD11c+ macrophages in PAT by immunofluorescence microscopy (n=6 per group). (D) The ratio of M1–M2 macrophages in PAT (n=6 per group). Data are presented as the mean±s.e.m. A two-tail Student's t-test was performed. P versus NP: ***P<0·001.