Figure 7.

Analysis of At1g17840 expression. A, Kinetics of salt, sugar, and ABA-responsive luciferase expression in line L0213. Three-week-old plants were transferred to MSAR media containing 250 mm NaCl, 400 mm Glc, or 50 μm ABA, and luminescence was recorded in 30-min intervals. B, RT-PCR analysis of At1g17840 expression. Total RNA was isolated from 3-week-old plants treated with 250 mm NaCl, 400 mm Glc, or 50 μm ABA for 1, 6, or 24 h. Equal amounts of cDNA templates were used for PCR amplification of gene-specific fragments using primers L213A and L213B. Upper row, At1g17840-specific PCR fragments are shown after 29 cycles of amplification (ABC). Lower row, PCR fragments corresponding to the ubiquitin 10 gene are shown after 23 cycles (UBI). C, Quantification of the PCR analysis. Average values of three measurements were normalized to control RNA.