Figure 1.

Description of the strategy used to generate the reference database of virtual tags. For each gene annotated in MIPS (26,620 in total), five different virtual cDNAs were obtained from the genome sequence by adding 100-, 200-, 300-, 400-, or 500-bp-long 5′- and 3′-UTRs to the predicted ORF. Two different virtual tag lists were generated from each of these classes of virtual cDNAs. The first list (Exclusive list) included only the virtual SAGE tag closest to the 3′ end of the virtual cDNA. The second list (Cumulative list) included all virtual SAGE tags located between the last MboI site in the ORF and the 3′ end of the virtual cDNA (two virtual tags in the example shown).