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Real-time reverse transcriptase (RT)-PCR quantification of the expression of several genes in the ucu1 and ucu2 mutants. The expression of AXR2 (A), IAA2 (B), ROT3 (C), and CPD (D) genes was studied in the presence of exogenous auxin (50 nm 2,4-D) or BR (100 nm 24-epibrassinolide) and compared with their expression under noninductive conditions in the wild type. Relative expression was normalized as regards to that of the OTC gene (see “Materials and Methods”).
