Table V.

Novel SSLP primer sets used in this work

Marker	BAC	Position in BAC	Oligonucleotide sequences (5′ → 3′)		PCR Product Size (bp)
			Forward labeled primer	Reverse primer	Ler	Col-0
AtMHC9.1	MHC9 (AP001305)	3,316—3,716	ACAGAGGAACAATTGATCTCATCa	TCACAGGAAACTCTGAGAAACG	448	400
AtMSD21.1	MSD21 (AB025634)	29,271—29,544	GTAGATATTATACATGTAACATTCGb	TTAAAAACGGTAAGGTAGTTATTAC	247	274
AtMZN24.2	MZN24 (AB028622)	69,687—69,707	ATCAACTGGAAGCACTTGCGCc	TGAGGTGTTTGCCGATCTACG	230	240
AtMW123.1	MW123 (AB022223)	52,271—52,588	GAAGCCTTAGTAATGCACCGGb	GATTGTGCATCCTTTGGCTGC	318d	354d

^aPrimer pair included an oligonucleotide labeled with 4,7,2′,4′,5′,7′-hexachlo-0-6-carboxyfluorescein phosphoramidites

^bPrimer pair included an oligonucleotide labeled with 6-carboxyfluorescein phosphoramidites

^cPrimer pair included an oligonucleotide labeled with 4,7,2′,7′-tetrachloro-6-carboxyfluorescein phosphoramiotes. Primers were designed based on the Col-0 genomic sequence data available at MIPS (http://mips.gsf.de/proj/db/index.html)

^dSizes were determined by sequencing the corresponding amplification products, which were found to contain 15 and nine TGGAGG repeats in the Col-0 and Ler accessions, respectively. In contrast to the Col-0 genomic sequence annotated at MIPS, which has been reported not to be different from that of Ler