Figure 2.

PmPLT1 and PmPLT2 can be expressed in yeast cells and catalyze the uptake of ¹⁴C-sorbitol. Transport rates for ¹⁴C-sorbitol were determined with the transgenic yeast cells MRYs1 and MRYas1 (expressing PmPLT1 in sense or antisense orientation) or with MRYs2 and MRYas2 (expressing PmPLT2 in sense or antisense orientation). The concentration of ¹⁴C-labeled sorbitol was 0.1 mm in all experiments. Where indicated, d-Glc was added to a final concentration of 10 mm.