Figure 4.

Transport properties of PmPLT1 and PmPLT2 in yeast cells. Transport of ¹⁴C-sorbitol (0.1 mm initial outside concentration) was analyzed in the presence of uncouplers (dinitrophenol or carbonyl cyanide-m-chlorophenylhydrazone) or in the presence of the SH-group inhibitor PCMBS. Inhibitors were added to a final concentration of 50 μm. Transport of 0.1 mm ¹⁴C-sorbitol was also analyzed in the presence of potential substrates added at a 100-fold excess (final concentrations 10 mm). Each bar results from at least three independent analyses (mean ± sd).