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Immunogold labeling of L. japonicus VDACs on root nodule sections. Nodules from 8-week-old L. japonicus nodules were used for immunogold labeling. Ultrathin sections (90 nm) were incubated with affinity-purified LjVDAC1.1 antibody. Secondary antibody was conjugated with 15-nm gold particles. Immunogold-labeled structures are marked with arrows. A, Small peripheral vesicles; B, mitochondria. SMs, bacteroids (B), polyhydroxybutyrate (PHB), and cell walls (CW) are also indicated.
