Figure 3.

Irreversible labeling for albumin expression using the dual lentivirus system (lineage tracing). (a) Schematic presentation of the lentivirus vectors. (b–d) Adult human liver cells or (e–g) β-TC1 cells were infected with the reporter virus in combination with ALB-Cre virus. Liver cells were imaged 10 days after infection for DsRed2 (red) or eGFP (green) autofluorescence (original magnification ×10). (h–k) Immunostaining of adult human cells infected with both viruses for Cre (blue, (j)), eGFP (green, (i)), and DsRed2 (red, (h)). (k) Merged image of all stainings. White arrows indicate DsRed2-positive cells. Yellow arrows indicate eGFP and Cre double positive cells. Original magnification ×60.