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. 2004 Jan;134(1):246–254. doi: 10.1104/pp.103.027466

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Copyright © 2004, The American Society for Plant Biologists

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Figure 3. — Recombinant ZM-INVINH1 inhibits invertase activity in vitro. ZM-INVINH1 produced in Escherichia coli was pre-incubated with an enzyme extraction from maize kernels harvested at 10 DAP. A, Invertase activity measured after pre-incubation with 20 to 200 pmol of recombinant ZM-INVINH1. B, Effect of Suc on ZM-INVINH1 inhibition of invertase activity. Pre-incubation of 10-DAP kernel enzyme extract with 50 pmol of ZM-INVINH1 and 1 or 5 mm Suc. The data are presented as a mean ± sd of three reactions.