Expression analysis of ZM-INVINH1 by RT-PCR. Top, Total RNA isolated from mature leaf (L), root (R), and tassel (T), as well as from whole kernels harvested 4, 7, 10, and 15 DAP was subjected to RT-PCR to amplify the ZM-INVINH1 transcript. A Mr marker (M) is included. Bottom, A control reaction using primers designed to amplify maize tubulin was also performed.