Figure 1.

Comparison of AtFd amino acid sequences with characterized Fd proteins. A, Protein sequences typical of leaf, root, and cyanobacterial [2Fe-2S] Fds aligned with Arabidopsis Fds in ClustalW 1.8 (http://searchlauncher.bcm.tmc.edu/multi-align/multialign.html) and compared in BOXSHADE 3.21 (http://www.ch.embnet.org/software/BOX-_form.html). Transit peptides are shown in lowercase and mature proteins in capitals. All transit peptide cleavage sites are as resolved through chemical sequencing of mature N termini (spinach [Spinacia oleracea] and radish [Raphanus sativus] available on http://www.ncbi.nlm.nih.gov; see Hase et al. [1991a] for maize sequences), except those of Arabidopsis, rice, and sweet orange, which were estimated by comparison with known mature proteins. White on black residues are those conserved in more than nine of the sequences analyzed. B, Fd phylogenetic tree. An alignment of mature Fd amino acid sequences, lacking transit peptides, was generated in ClustalW 1.8 as for A. This alignment is presented as a Phylogram.