Physical properties of AtFd proteins. A, Separation of purified mature recombinant proteins. Left panel, SDS-PAGE (1 μg protein lane-1); right panel, native gradient PAGE (20 μg lane-1). Lanes were 1, AtFd1; 2, AtFd2; 3, AtFd3; and 4, AtFd4. B, Absorption spectra: AtFd1, bold gray; AtFd2, bold black; AtFd3, light gray; and AtFd4, dark gray.