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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Biotechniques. 2011 Aug;51(2):119–128. doi: 10.2144/000113720

Figure 4.

Figure 4

Effect of the IRES-SVLT-SV40 ori elements on mammalian two-hybrid interaction analysis between Id and MyoD using gal4-responsive reporter genes. HEK293 cells were transiently co-transfected with indicated combinations of pBIND-Id and pACT-MyoD and either the standard control (TK-Luc) or gal4-responsive (TK-Gal-Luc) reporter genes (A) or the corresponding reporter genes containing the IRES-SVLT elements (TK-Luc-IRSV and TK-Gal-Luc-IRSV) (B). Luciferase activities reported as relative luminescence units (RLU) are shown (mean±s.d.; n=4) and are indicative of at least three independent experiments.