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The effect of FT-EtOH extract on MAPK-mediated signaling pathways. (a) K562 cells were treated with FT extract for 10 min. Cells were lysed and analyzed by western blotting with antibodies probing the phosphor- and total forms of p38, ERK and SAPK. (b) K562 cells pre-treated with p38-inhibitor SB203580 (SB) for 10 min in the presence of FT-EtOH extract for 10 min. Cells were lysed and analyzed by western blotting. Similar results were obtained in three separate experiments.
